Thermo Scientific™ LweI (SfaNI) (10 U/μL)

The LweI (SfaNI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: SfaNI).

Product Code	Brand	Quantity
10252410	Thermo Scientific™ ER1621	100 U
10389899	Thermo Scientific™ ER1622	500 U

Description

Lambda DNA digested with LweI (SfaNI), 1.4% agarose, 169 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality -stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Specifications

• Concentration: 10 U/μL
• Enzyme: LweI (SfaNI)
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: Yes
• Research Category: Scarless Cloning

• Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
• Compatible Buffer: 10X Buffer Tango
• Optimal Reaction Temperature: 37°C
• Product Type: Restriction Enzyme

For Research Use Only. Not for use in diagnostic procedures.