Thermo Scientific™ pJET1.2 Forward Sequencing Primer, 23-mer

Thermo Scientific sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends.

Brand:  Thermo Scientific™ SO501

Product Code. 10590091

Description

Thermo Scientific sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends. pJET1.2 sequencing primers flank the Eco32I site in the eco47IR gene of positive selection cloning vector pJET1.2. All primers are supplied as 10 µM aqueous solutions.

Applications
• Sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2p sequence
• Colony screening by PCR

pJET1.2 Primer sequences
• pJET1.2 forward sequencing primer, 23-mer: 5'-d(CGACTCACTATAGGGAGAGCGGC)-3'
• pJET1.2 reverse sequencing primer, 24-mer: 5'-d(AAGAACATCGATTTTCCATGGCAG)-3'

Related products
pJET1.2 Reverse Sequencing Primer, 24-mer (Cat. No. SO511)

Specifications

• Product Type: Forward Sequencing Primer
• Shipping Condition: Dry Ice
• Primer: pJET
• For Use With (Application): Sequencing

• Content And Storage: T3 promoter Sequencing Primer, 24-mer, 10 μM
  
  Store at –20°C.
• Concentration: 10 μM
• Vector: pJET1.2
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.