Thermo Scientific™ pTZ19R DNA

Perform DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system with this double-stranded closed circular high copy phagemid DNA.

Brand:  Thermo Scientific™ SD0141

Product Code. 10629900

Description

pTZ19R/U are small phagemids 2862 bp in length, constructed by inserting the DNA of phage f1 intergenic region (IG) into pUC19 and the T7 promoter sequence near to the MCS of pUC19. The phagemids differ in the orientation of the cloned f1 IG region. They are designed for DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system. A host strain harboring these phagemids will produce single-stranded DNA if superinfected with the helper phage M13K07.

Highlights

• Isolated from E. coli (dam+, dcm+)
• More than 90% in the supercoiled form
• Isolated from E.coli (dam+, dcm-)

Specifications

• Cloning Method: Restriction Enzyme⁄ MCS
• Sample Type: DNA
• For Use With (Application): Cloning
• Quantity: 50 μg

• Concentration: 0.5 μg/μL
• Final Product Type: DNA
• Product Type: DNA
• Vector: pTZ19R

For Research Use Only. Not for use in diagnostic procedures.