Fisher BioReagents™ Taq DNA Polymerase

Allows up to 100 cycles of amplification without loss of activity

Brand:  Fisher BioReagents™ FB6000-75

Product Code. 10104974

Description

• Licensed for use in PCR
• Uses a thermostable DNA polymerase that has been encoded by a modified form of Thermus aquaticus DNA polymerase gene that has been inserted into an E. coli host
• Has a 5«→3« exonuclease activity
• Available with two different reaction buffers
• Buffer A contains an optimized final concentration of 15mM MgCl₂ for most PCR amplifications
• Buffer B does not contain MgCl₂; MgCl₂ is provided in a separate vial as a 25mM solution
• Storage Buffer: 20mM Tris-HCl, pH 7.5 (at room temperature), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Tween™ 20, 0.5% Nonidet P40™
• Recommended Assay Buffer: 10X Assay Buffer A (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl; 15mM MgCl₂) or 10X Assay Buffer B (100mM Tris-HCl, pH 9.0 [at 25°C/77°F]; 500mM KCl) and 25mM MgCl₂ solution in a separate vial

Unit Definition: One unit of enzyme is defined as the amount that will incorporate 10nmol of dNTPs into acid-insoluble material per 30 minutes in a 10-minute incubation at 74°C (165°F) under the assay conditions above

Specifications

• Composition: 10X assay buffer B : Tris-HCl 100mM, pH 9 at 25°C, KCl 500mM and MgCl₂ 25mM solution in a separate vial
• Description: Taq DNA Polymerase
• Format: Poly Tube
• For Use With (Application): Standard PCR
• Storage Buffer: Tris-HCl 20mM, pH 7.5 (at room temperature), KCl 100mM, EDTA 0.1mM, DTT 1mM, Glycerol 50%, Tween™ 20 0.5%, Nonidet™ P40™ 0.5%

• Content And Storage: -20°C
• Form: Liquid
• Quantity: 5 × 2500 Units
• Concentration: 5U/μL