Thermo Scientific™ FL-AR N-TERM Peptide

LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.

Brand:  Thermo Scientific™ PV4616

Product Code. 10515295

Description

LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:

LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers. Store peptides at -20°C.

Specifications

• Purity: ≥96% by HPLC
• Content And Storage: 100 μM Fluorescein-AR N-term Peptide in 99.9% DMSO, 0.1% formic acid.
  
  Store at -20°C and protect from light.
• For Use With (Application): Pharma and biopharma, target and lead identification and validation, drug discovery and development, nuclear receptor biology, nuclear receptor proteins, biochemical nuclear receptor assays, nuclear receptor coregulator interaction
• Molecular Weight (g/mol): 2602Da
• Quantity: 100 μL
• Sequence: Fluorescein-SKTYRGAFQNLFQSVREVI
• Storage Buffer: 99.9% DMSO, 0.1% formic acid

• Common Name: Fluorescein - AR N-TERM Peptide
• Description: Fluorescein-AR N-TERM Peptide, 100μL, 2602Da Molecular Weight, –5 to –30°C Storage Condition, Used for Pharma and Biopharma, Target and Lead Identification and Validation
• Label Type: Classic Dyes
• Product Type: Fluorescein - AR N-TERM Peptide
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Shipping Condition: Dry Ice

For Research Use Only. Not for use in diagnostic procedures.