Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Fluo-4 Direct™ Calcium Assay Kit

Product Code	Brand	Quantity
10297783	Molecular Probes™ F10471	100 ml
10033614	Molecular Probes™ F10472	100 ml
10637215	Molecular Probes™ F10473	1000 ml

Description

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

Specifications

• Color: Green
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
• Emission: Visible
• Modification: Chemical
• Shipping Condition: Room Temperature

• Detection Method: Fluorescence
• Dye Type: Fluorescent Dye-Based
• Form: Powder
• Product Line: Molecular Probes™
• Sub Cellular Localization: Nucleus, Organelles, Cytoplasm

For Research Use Only. Not for use in diagnostic procedures.