Gibco™ Freedom™ CHO-S™ Kit

Freedom™ CHO-S™ Kit

Brand:  Gibco™ A1369601

Product Code. 11370812

Description

Gibco™ Freedom™ CHO-S™ Kit, co-developed with ProBioGen AG, is an easy-to-use, beginning-to-end product, for cloning and expression of recombinant proteins in Chinese Hamster Ovary (CHO)-derived suspension culture. The Gibco™ Freedom™ CHO-S™ Kit includes components for transfection, expression, clone creation, and stable cell line selection, all in a conveniently packaged kit. The Gibco™ Freedom™ CHO-S™ Kit also delivers high titers, and offers simplified commercial licensing options.

• Flexible commercial licensing without the need for royalties
• Ability to achieve IgG titers over 3g/L
• Complete workflow that can take your gene of interest from transfection to lead clone typically in 5 months
• Convenient packaging, for simplified ordering and storage
  
  Commercial Licensing that is Simple, Flexible and Without Royalties
  Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco™ Freedom™ Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at outlicensing@lifetech.com.
  
  Industry Standard Production Levels
  Current bioprocess industry standards to achieve manufacturing efficiencies for IgG type molecule production with stable CHO cell lines in bioreactors is 2-3g/L or better. We have demonstrated that the Gibco™ Freedom™ CHO-S™ Kit can consistently generate IgG producing scalable clones (stable out to 60 population doublings), that meet this standard of productivity (Fig 2).
  
  Complete, Fast, and Efficient Workflow
  The Gibco™ Freedom™ CHO-S™ Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in 5 months, with reduced hands on time, and reduced Fulltime Equivalent (FTE) requirements (Fig 3). The entire workflow is performed in our CD FortiCHO™ medium, a chemically defined, animal origin free (AOF) medium. The kit also offers the following advantages:
   
• Cloning of one or two genes that encode your protein(s) of interest
• High efficiency transfection of DNA into CHO-S™ Cells (cGMP-banked)
• Included flash drive with loaded manual
  
  Access to Gibco™ Freedom™ Kit Support
  If any specific support is needed beyond the manual, please contact your account manager to obtain a Process Science Manager consultation. In addition, one of our support scientists may check in with you to ensure that your experience with the kit is as successful as possible. For further assistance, please email our support team at GibcoServices@thermofisher.com.

Specifications

• Cell Type: CHO-S Cells, cGMP Banked
• Delivery Type: Transfection
• Promoter: CMV/EF1 Hybrid Promoter, EF2/CMV Hybrid Promoter
• Selection Agent (Eukaryotic): Puromycin, MTX (Methotrexate)

• Content And Storage: CD FortiCHO™ Medium: 2°C to 8°C, protect from light
  OptiPRO™ SFM: 2°C to 8°C, protect from light
  FreeStyle™ MAX Reagent: 2°C to 8°C, do not freeze
  
  CHO–S™ Cells (cGMP–banked): liquid nitrogen
  One Shot™ TOP10 Chemically Competent E. coli: –80°C
  Freedom™ pCHO 1.0 Expression Vector: –5°C to –20°C
• Key Functions: Protein Production, Stable Cell Line Development
• Quantity: 1 Kit
• Selection Marker Eukaryotic: DHFR, PurR

For Research Use Only. Not for use in diagnostic procedures.