GATA3 Monoclonal Antibody (7B5), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA515641

Product Code. 11395603

Description

MA5-15641 targets GATA3 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15641 immunogen is purified recombinant fragment of human GATA3 expressed in E. Coli. <br>. MA5-15641 detects GATA3 which has a predicted molecular weight of approximately 48kDa.

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

Specifications

• Antigen: GATA3
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: ascites with 0.03% sodium azide
• Gene Accession No.: P23771
• Gene Symbols: GATA3
• Immunogen: Purified recombinant fragment of human GATA3 expressed in E. Coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 2625
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Western Blot
• Clone: 7B5
• Conjugate: Unconjugated
• Gene: GATA3
• Gene Alias: EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1