Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Brand:  Invitrogen™ A21177

Product Code. 15515180

Includes:

• CRISPR CD4 Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control Double-stranded Oligonucleotide
• OneShot™ TOP10 Chemically Competent Cells

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. Competent cells included for added convenience and highest cloning efficiencies.

GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).


The GeneArt™ CRISPR Nuclease Vector system with competent cells offers

• Easy-to-design genome engineering system
• Affordable, ready-to-use cloning vectors
• Enrichment for hard to transfect or difficult to modify cell lines

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

• Cloning Method: Restriction Enzyme/MCS
• Detection Method: Primer-probe
• Format: Kit
• Technique: CRISPR-Cas9
• Product Type: Crispr Nuclease Vector With CD4 Enrichment Kit
• Protein Tag: CD4 fusion
• Sufficient For: 10 Reactions
• No. of Reactions: 10 Reactions
• Starting Material: Oligos (DNA)

• Content And Storage: • CRISPR CD4 Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control, double stranded oligonucleotide
  Store the above at -5 to -30°C.
  
  • OneShot™ TOP10 Chemically Competent Cells
  Store at -80°C.
• Form: Liquid
• Reaction Speed: Fast
• Product Line: GeneArt™
• Promoter: U6, CMV
• Reporter Gene: CD4
• For Use With (Application): Gene Expression, Cloning
• Overhang: 3' overhangs

For Research Use Only. Not for use in diagnostic procedures.