Invitrogen™ GeneArt™ Linear pUC19L Vector for Seamless Cloning

A ready-to-use, linear version of the popular cloning vector pUC19

Brand:  Invitrogen™ A13289

Product Code. 10343030

Description

The GeneArt™ Linear pUC19L Vector is a ready-to-use, linear version of the popular cloning vector pUC19. The pUC19L plasmid (2.7 Kbp), is specifically designed as a cloning and DNA assembly vector for the GeneArt™ Seamless Cloning and Assembly Kit (cat # A13288). The vector is manufactured by digesting supercoiled pUC19 plasmid with the restriction enzymes PstI and KpnI, and tested for cloning of up to 4 DNA fragments.

• Some of the key features and elements of the pUC19L vector include:
• lacZα gene allows easy blue⁄white colony screening for selection of recombinants
• pUC origin of replication (high copy vector)
• Ampicillin resistance for selection in E. coli
• 6 convenient and validated restriction sites flanking the cloning region
• Tested for functionality by assembly with 4 DNA fragments using the GeneArt™ Seamless Cloning and Assembly Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Content And Storage: Store the product at -80°C. The vector is guaranteed stable for 6 months when properly stored.
• For Use With (Application): PCR cloning, seamless cloning and genetic assembly
• No. of Reactions: 20
• Quantity: 20 Reactions
• Vector: Seamless Cloning Vector, Linear

• Description: Reporter: Beta-Gal (lacZ)
• Includes: The product contains 5 vials of linear pUC19L vector at a concentration of 50ng⁄ml. Each vial contains enough plasmid for 4 cloning reactions using the GeneArt™ Seamless Cloning and Assembly Kit (A13288 - must be purchased separately).
• Product Type: GeneArt™ Linear pUC19L Vector for Seamless Cloning
• Sample Volume: 13 kb total (vector plus all inserts), Up to 4 Fragments