GFP tag Mouse anti-Tag, HRP, Clone: 1E10H7, Proteintech

Mouse Monoclonal Antibody

Brand:  Proteintech HRP-66002-100UL

Product Code. 16861246

Description

This antibody is conjugated with HRP. Note: Do not add azide (Sodium Azide or Smite) into the dilution buffer. azide is the HRP inhibitor which decreases the enzymatic activity of HRP.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressing and protein localization. Moreover, other applications for GFP include its use in assessing protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP tag
• Classification: Monoclonal
• Concentration: 1.33 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% thimerosal; pH 7.3
• Host Species: Mouse
• Quantity: 100 μL
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot
• Clone: 1E10H7
• Conjugate: HRP
• Gene Alias: CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Tag