Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Brand: Gibco™ 12491015
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Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.
This Advanced DMEM is manufactured as follows:
With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine
The complete formulation is available.
Using Advanced DMEM
Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Shipping Condition: Room Temperature
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
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| Animal Origin | |
| Liquid | |
| Reduced Serum | |
| Sterile-filtered | |
| No Glutamine, No HEPES | |
| Gibco™ | |
| Room Temperature |
| MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA) | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 2715552 | Certificate of Analysis | 29/01/2025 | 12491015 |
| 2809606 | Certificate of Analysis | 28/01/2025 | 12491015 |
| 2773607 | Certificate of Analysis | 28/01/2025 | 12491015 |
| 2832351 | Certificate of Analysis | 28/01/2025 | 12491015 |
| 2843048 | Certificate of Analysis | 28/01/2025 | 12491015 |
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For Research Use Only. Not for use in diagnostic procedures.
