Gibco™ Advanced DMEM/F-12

Product Code. 11540446

Brand: Gibco™ 12634010

Description

Requires

Supplementation

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:

With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Using Advanced DMEM/F-12

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: MRC-5, SP2, Vero, WI-38 and Jurkat
• Classification: Animal Origin
• Concentration: 1 X
• Form: Liquid
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
• Serum Level: Reduced Serum
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
• Without Additives: No Glutamine, No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Safety and Handling

Identifiant produit

• Methyl benzoate

Mention d'avertissement

• Warning

Catégorie de danger

• Acute toxicity Category 4

Mention de danger

• H302-Harmful if swallowed.

Conseils de prudence

• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.

Informations supplémentaires

• MIXTURE LIST-Contains : Methyl benzoate

For Research Use Only. Not for use in diagnostic procedures.