Gibco™ Basal Medium Eagle (BME)

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells.

Brand:  Gibco™ 21010046

Product Code. 15470604

Description

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

This BME is manufactured as follows:

• With: Phenol Red
• Without: L-glutamine, HEPES

The complete formulation is available.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO₂ incubator.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Line: HeLa, mouse fibroblasts, WI-38 and MRC-5
• Concentration: 1 X
• Product Type: Basal Medium Eagle (BME)
• With Additives: Low Glucose, Phenol Red
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.