Gibco™ DMEM, powder, high glucose, pyruvate

Artikelnummer. 11588826

Marke: Gibco™ 12800017

Beschreibung

Benötigt

Supplementation

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red, Sodium Pyruvate

Without: HEPES, Sodium Bicarbonate

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Bestellinformation

Shipping Condition: Room Temperature

Spezifikation

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Classification: Animal Origin-free
• Form: Powder
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Without Additives: No HEPES, No Sodium Bicarbonate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco
• Quantity: 10 x 1 L
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8° C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.