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Gibco™ Ham's F-10 Nutrient Mix
GREENER_CHOICE

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells.

Brand:  Gibco™ 11550043

23.04 GBP valid until 2024-11-29
Use promo code "21683" to get your promotional price.


Product Code. 15490554

  • £24.25 / 500mL

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Requires : Supplementation
This item is not returnable. View return policy

Description

Description

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

This Ham's F-10 is modified as follows:
With: Phenol Red, L-glutamine
Without: HEPES

The complete formulation is available.

Using Ham's F-10
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Rat Astrocytes
1 X
Ham's F-10 Nutrient Mixture
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Sustainable packaging
Gibco™
Room Temperature
CHO, COS-7, and rat prostate epithelial cells
Animal Origin-free
Liquid
Sterile-filtered
No HEPES
cGMP-compliant under the ISO 13485 standard
500 mL
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For Research Use Only. Not for use in diagnostic procedures.