Gibco™ Ham's F-12K (Kaighn's) Medium

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

Brand:  Gibco™ 21127030

Product Code. 10667440

Description

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using Ham's F-12K

Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 500 mL

• Cell Type: Human Hepatocytes
• Concentration: 1 X
• Product Type: Ham's F-12K (Kaighn's) Medium
• Sterilization Method: Sterile-filtered
• Without Additives: No HEPES
• Product Line: Gibco™
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.