Gibco™ Ham's F-12K (Kaighn's) Medium

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

Brand:  Gibco™ 21127022

Product Code. 11580556

Description

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using Ham's F-12K

Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8°C. Protect from light
  Shipping conditions: Ambient
  
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL

• Cell Type: Human Hepatocytes
• Concentration: 1 X
• Product Type: Ham's F-12K (Kaighn's) Medium
• Sterilization Method: Sterile-filtered
• Without Additives: No HEPES
• Product Line: Gibco™
• Shipping Condition: Room Temperature

Safety and Handling

Product Identifier

• Silver chloride

Signal Word

• Warning

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 1
• ACUTE AQUATIC Acute 1
• Substances and mixtures corrosive to metals Category 1

Hazard Statement

• H290-May be corrosive to metals.
• H400-Very toxic to aquatic life.
• H410-Very toxic to aquatic life with long lasting effects.

Precautionary Statement

• P273-Avoid release to the environment.
• P390-Absorb spillage to prevent material damage.
• P391-Collect spillage.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Silver chloride

For Research Use Only. Not for use in diagnostic procedures.