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Gibco™ Medium 199, Earle's Salts
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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Brand:  Gibco™ 11150059

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Product Code. 15440554

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Description

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Storage conditions: 2–8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
1X
Medium 199
Sterile-filtered
No HEPES, No Sodium Pyruvate
Gibco™
Room Temperature
Rat epithelial
Animal Origin
Liquid
Sterile-filtered
Low Glucose, Glutamine, Phenol Red
cGMP-compliant under the ISO 13485 standard
500 mL
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.