Gibco™ RPMI, no methionine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ A1451701

Product Code. 12857552

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using our media selector tool.

This RPMI medium is modified as follows:
With: L-glutamine, Phenol Red
Without: Methionine, HEPES

Gibco™ RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Certifications

ISO 13485

Specifications

• Amino Acids: No Methionine
• Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Glutamine, Phenol Red, Sodium Bicarbonate
• For Use With (Application): Mammalian Cell Culture
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 500 mL
• Shipping Condition: Room Temperature

• Content And Storage: Storage conditions: 2-8 Deg;C. Protect from light
  
  
  
  Shipping conditions: Ambient
  
  
  
  Shelf life: 12 months from date of manufacture
• Cell Type: Leukemic Cells
• Concentration: 1 X
• Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Sodium Pyruvate
• Green Features: Sustainable packaging
• Product Line: Gibco™
• Regulatory Status: RUO