Goat anti-Human IgG Fc Recombinant Secondary Antibody, Alexa Fluor™ 488, Invitrogen™

Goat Recombinant Monoclonal Secondary Antibody

Product Code	Brand	Quantity
17876752	Invitrogen A5574750UL	50 μL
17895972	Invitrogen A55747	250 μL

Description

This Goat anti-Human IgG Fc Recombinant Secondary Antibody specifically detects human IgG Fc with no cross-reactivity to human IgA or IgM or various species immunoglobulins. The sensitivity and specificity of each lot is confirmed using ELISA. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG Fc
• Concentration: 1.0 mg/mL
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 5mM sodium azide; pH 7.4
• Isotype: IgG
• Purification Method: Protein G
• Product Type: Secondary Antibody

• Clone: 3H8L9
• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Conjugate: Alexa Fluor 488
• Host Species: Goat
• Target Species: Human
• Immunogen: Purified Human IgG
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!