Goat anti-Human IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 647, Invitrogen™

Goat Recombinant Polyclonal Secondary Antibody

Product Code	Brand	Quantity
17834574	Invitrogen A5601950UL	50 μL
17844594	Invitrogen A56019	250 μL

Description

This Goat-anti-Human IgG (H+L), Superclonal Recombinant Secondary Antibody specifically detects human IgG Heavy and the Light chain with no cross-reactivity to other species immunoglobulins except moderate cross-reactivity towards Monkey IgG. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency. Superclonal Recombinant antibodies are a defined pool of recombinant monoclonal antibodies, manufactured by selecting and pooling individual clones to provide complementary, specific, and optimal epitope coverage to achieve enhanced performance. The sensitivity and specificity of each lot is confirmed using ELISA. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (H+L)
• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Conjugate: Alexa Fluor Plus 647
• Host Species: Goat
• Target Species: Human
• Immunogen: Purified Human IgG
• Primary or Secondary: Secondary
• Content And Storage: 4° C, store in dark

• Concentration: 1.0 mg/mL
• Classification: Recombinant Polyclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.5
• Isotype: IgG
• Purification Method: Affinity chromatography