Goat anti-Human IgG1 (Heavy chain) Recombinant Secondary Antibody, HRP, Invitrogen™

Goat Recombinant Monoclonal Secondary Antibody

Product Code	Brand	Quantity
17888801	Invitrogen A5573950UL	50 μL
17848801	Invitrogen A55739	250 μL

Description

This Goat anti-Human IgG1 (Heavy chain) Recombinant Monoclonal Antibody specifically detects human IgG1 heavy chain with no cross-reactivity to human IgG2, IgG3, IgG4, IgA or IgM or various species immunoglobulins, including monkey IgG. The sensitivity and specificity of each lot is confirmed using ELISA. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG1 (Heavy chain)
• Concentration: 1.0 mg/mL
• Classification: Recombinant Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with proprietary stabilizer and proprietary preservative; pH 7.4
• Isotype: IgG
• Purification Method: Protein G
• Product Type: Secondary Antibody

• Clone: 3H60L9
• Applications: ELISA, Peptide Array, Western Blot
• Conjugate: HRP
• Host Species: Goat
• Target Species: Human
• Immunogen: Purified Human IgG
• Primary or Secondary: Secondary
• Content And Storage: 4° C, do not freeze