Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488, Invitrogen™

Goat Recombinant Polyclonal Secondary Antibody

Brand:  Invitrogen A28175SAMPLE

Product Code. 15607878

Description

The sensitivity and specificity of each lot is confirmed using ELISA. Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L)
• Classification: Oligoclonal
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 5mM sodium azide
• Immunogen: Recombinant full-length protein
• Quantity: 50 μg
• Primary or Secondary: Secondary
• Includes: 5mM Sodium azide
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot
• Concentration: 1 mg/mL
• Dilution: 1 to 5μg/mL, 0.1 to 2μg/mL, 1:1000
• Host Species: Goat
• Purification Method: Gravity column chromatography
• Regulatory Status: RUO
• Packaging: 1 Each
• Target Species: Mouse
• Form: Liquid