Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP, Invitrogen™

Goat Polyclonal Secondary Antibody

Brand:  Invitrogen 31460

Product Code. 11859140

Description

Product # 31460 has been successfully used in Western blot, IHC and IP applications. Product # 31460 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock™ Blocking Buffers. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. Reconstitute with 2.0 mL of distilled water (0.8 mg/mL after restoration).

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L)
• Classification: Polyclonal
• Conjugate: HRP
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Rabbit
• Form: Lyophilized

• Applications: ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 0.8 mg/mL
• Formulation: PBS with 15mg/mL BSA, 50mM sucrose and no preservative; pH 7.6
• Immunogen: Purified Rabbit IgG, whole molecule
• Quantity: 2 mL
• Primary or Secondary: Secondary
• Content And Storage: 4° C
• Isotype: IgG