Thermo Scientific Chemicals Guanidine hydrochloride, Molecular Biology Grade

Description

A protein denaturant

Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

Solubility
Freely soluble in water

Notes
Protect from heat. Store away from oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 50-01-1
• Molecular Formula: CH6ClN3
• Molecular Weight (g/mol): 95.53
• MDL Number: MFCD00013026
• InChI Key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Synonym: guanidine hydrochloride, guanidinium chloride, guanidine, monohydrochloride, aminoformamidine hydrochloride, aminomethanamidine hydrochloride, guanidine monohydrochloride, guanidine hcl, guanidine chloride, iminourea hydrochloride, carbamidine hydrochloride
• PubChem CID: 5742
• ChEBI: CHEBI:32735
• IUPAC Name: guanidine;hydrochloride
• SMILES: C(=N)(N)N.Cl

• Melting Point: 178°C to 185°C
• Color: White
• Density: 1.34
• Quantity: 100 g
• Beilstein: 3591990
• Sensitivity: Hygroscopic
• Merck Index: 144562
• Solubility Information: Freely soluble in water
• Formula Weight: 95.53
• Grade: Molecular Biology
• Physical Form: Crystalline Solid
• Chemical Name or Material: Guanidine hydrochloride

Safety and Handling

Product Identifier

• Guanidine hydrochloride, Molecular Biology Grade

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Guanidine hydrochloride

RUO – Research Use Only