Invitrogen™ HCK Human Protein

Easily screen for kinase binding. Thermo Fisher Invitrogen™ HCK Human Protein fusion protein consists of the catalytic domain of transforming growth factor beta receptor 2 (TGFBR2) and glutathione S-transferase (GST) tag. It is validated in the LanthaScreen™ Eu Kinase Binding Assay.

Product Code	Brand	Quantity
11330872	Invitrogen™ PV6128	10μg
15418723	Invitrogen™ PR9171A	100μg
15438813	Invitrogen™ PV6129	1mg

Description

The LanthaScreen™ Eu Kinase Binding Assay is a TR-FRET assay that lets you measure inhibitor binding to non-activated kinases, perform simple off-rate measurements and makes simple the study of type III (allosteric) inhibitors.

The LanthatScreen™ Eu Kinase Binding Assay is easy to optimize and run. The assay uses a simple mix-and-read format with a single one-hour incubation and no development steps. Simultaneous binding of an Alexa Fluor™ conjugate or tracer” to a tagged kinase is detected by addition of a europium-labeled anti-tag antibody, in this case anti-GST. Simultaneous binding of the tracer and antibody to the kinase results in a high degree of fluorescence resonance energy transfer (FRET), while displacement of the tracer with a kinase inhibitor results in a loss of FRET.

• Compatible and validated with the LanthaScreen™ Eu Kinase Binding Assay
• Expressed in insect cells
• GST tagged for use with Eu-labeled GST antibody
• No special measures were taken to activate this kinase

TGFBR2 for the LanthaScreen Kinase Binding Assay
Transforming Growth Factor, beta receptor 2 (TGFBR2) is a member of the TGF-beta family of receptor Serine/Threonine kinases. It is involved in the regulation of cell proliferation. (TGFBR2 Aliases: AAT3, FAA3, MFS2, RIIC, LDS1B, LDS2B, TAAD2, TGFR-2, TGFbeta-RII).

This insect cell-expressed recombinant fusion protein of the TGFBR2 catalytic domain and GST tag has been validated in the LanthaScreen™ Eu Kinase Binding Assay with the Tracer 236 and Eu-labeled GST Antibody reagents.

Specifications

• Accession Number: NP_002101
• Molecular Weight (g/mol): 84.8kDa
• Structural Form: Full Length
• KinaseFamily: SRC Kinase Family
• Recombinant: Recombinant
• Expression System: Baculovirus

• Formulation: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% NP-40, 2mM DTT and 50% glycerol.
• Storage Requirements: Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Gene Alias: HCK
• Species: Human
• Protein Tag: GST Tag
• Form: Frozen