Cytiva His Mag Sepharose™ excel Chromatography Media

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Product Code	Brand	Quantity
12642966	Cytiva 17371222	10 x 1 mL
12601216	Cytiva 17371221	5 x 1 mL
12691206	Cytiva 17371220	2 x 1 mL

Description

Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.

• His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
• The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
• Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
• Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
• Increase target protein yield and decrease degradation through reduced and simplified sample handling.
• Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
• Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
• Simplify handling using visible and dense Sepharose-based magnetic beads.
• Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
• The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
• The base matrix is based on Sepharose medium with magnetite incorporated.
• His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.

Specifications

• pH Range: 2 to 12
• Content And Storage: 4°C to 30°C
• For Use With (Application): Designed to simplify small-scale purification and screening of His-tagged Proteins_x000D_ secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma

• Support Type: Highly cross-linked spherical Agarose including Magnetite
• Particle Size: 63 μm
• Type: Chromatography Media

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains Nickel. May produce an allergic reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN AQUATIC-Contains 50% of components with unknown hazards to the aquatic environment.