Cytiva HisPrep Column

Optimize purification of histidine-tagged recombinant proteins. Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.

Brand:  Cytiva 28-9365-51

Product Code. 10267934

Description

• Optimized for convenient scale-up purification of histidine-tagged proteins
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
• Negligible leakage of Ni2+
• Bed Volume 20ml
• High binding capacity
• Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium

• Media:
• BioProcess media
• 90μm average particle size
• Metrix 6% cross-linked agarose
• pH stability working range 3-12¹
• Metal ion capacity ∽15μmol Ni2+™/ml medium
• Three years shelf life
• Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3

• Column:
• Inside Diameter is 16mm
• Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
• Bed support Filter

• Bed Dimensions 16 x 100mm
• Flow rate <10ml/min¹
• Flow Velocity <300cm/h
• Pressure Max. 1.5 bar

Specifications

• Diameter (English) Inner Column: 0.6 in.
• Content And Storage: 4°C to 30°C
• Volume (Metric): 20 mL

• Flow Rate: <10 mL/min.
• Diameter (Metric) Inner Column: 1.6 cm
• Quantity: Each

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• EUH208-Contains . May produce an allergic reaction.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Nickel