Invitrogen™ Hoechst 33342 Ready Flow™ Reagent

Hoechst 33342 Ready Flow™ Reagent is a bright, easy-to-use cell-permeant stain for cell cycle analysis on a flow cytometer.

Brand:  Invitrogen™ R37165

Product Code. 15932344

Description

This dye emits blue fluorescence when bound to double-stranded DNA with an emission maximum at 461 nm.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Cell-permeable and effective in fixed and live-cell staining for cell cycle

Hoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 10⁶ cells, incubate, and analyze.

Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. This dye binds into the minor grooves of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. Hoechst 33342 Ready Flow Reagent is excited by UV light and has peak emission at 460 nm.

Specifications

• Color: Blue
• Excitation Wavelength Range: 361/497
• Quantity: 120 assays
• Product Type: Nucleic Acid Stain

• Content And Storage: 2 dropper bottles, store at room temperature
• Dye Type: Cell-Permeant
• Product Line: Ready Flow™
• SubCellular Localization: Nucleus