Invitrogen™ Human Cathepsin C ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EH73RBX10

Product Code. 17767843

Description

Cathepsin C, known also as dipeptidyl aminopeptidase I (DPPI), is a tetrameric lysosomal cysteine peptidase belonging to the papain family. Cathepsin C is involved in intracellular protein degradation and the processing of protein precursors, where it participates in cell growth, neuraminidase activation, and platelet factor XIII activation. Cathepsin C is largely related to other lysosomal cysteine proteinases, including cathepsin B, H and L. Enzymatically, Cathepsin C is capable of sequentially removing dipeptides from the amino terminus, and it requires halide ions, namely chloride ions, and thiols for complete enzymatic activity. Protein levels of Cathepsin C are detected in a variety of tissues, and it is most highly expressed in spleen, kidney, cytotoxic lymphocytes and myeloid cells, where it localizes to the secretory granule compartment. Cathepsin C is initially synthesized as a proenzyme that is rapidly processed to generate two distinct chains that function together as the mature form of the enzyme.

Specifications

• Accession Number: P53634
• Assay Sensitivity: 2 ng/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CTSC
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 2 to 500 ng/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): 1075
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate|Standard|Assay Diluent concentrate|Biotinylated Detection Antibody|SAV-HRP|Wash Buffer|Chromogen|Stop Solution|Adhesive Plate Covers
• Quantity: 10 x 96 Tests
• Sample Volume: Serum, 50 μL|Plasma, 50 μL|Supernatant, 50 μL
• Target: Cathepsin C
• Total Assay Time: 4 hr 45 min