Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit

Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.

Brand:  Invitrogen™ 411101

Product Code. 10531594

Description

Assay IFN-a (human interferon alpha) levels in human serum, plasma and tissue culture media with these Thermo Scientific™ Human IFN alpha ELISA Kits (for Serum Samples).

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related,species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans,the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities),and single beta,omega,epsilon and kappa isoforms. Homologous molecules are found in many species,including rats and mice (and most mammals),and have been identified in birds,reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells,is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Assay Range: 12.5-1000pg/mL
• Conjugate: HRP
• Product Type: ELISA Kit
• Specificity: No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t
• Target Species: Human
• Gene Symbol: IFNA1,IFNA2
• Intraassay CV: <8%
• Label or Dye: HRP
• Precision: Intra-assay CV ≤ 8%; inter-assay CV ≤ 8%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Description: Human IFN alpha ELISA Kit (Serum Samples)
• Immunoassay Kit Format: Colorimetric ELISA (TMB substrate) with Plates
• Sample Type: Supernatant
• Sufficient For: 96 microplate wells
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <8%
• Kit Contents: Wash Solution Concentrate, 50mL, Sample Diluent, 30mL, Antibody Concentrate, 1 vial, Concentrate Diluent, 15mL, Dilution Buffer, 30mL, HRP Conjugate Concentrate, 1 vial, Human IFN Alpha Standard 10,000pg/mL, 1 vial, Precoated 96-well Plate, 1 ea, Stop Solution, 15mL, TMB Substrate Solution, 15mL, Plate Sealers, 4 ea
• Measurement: Absorbance at 450nm
• Quantity: 96 Tests
• Sample Volume: Supernatant, 100 μL
• Target: Human
• Total Assay Time: 3 hr. 15 min.