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Invitrogen™ IL-21 Human Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of IL-21 protein levels from samples including serum, plasma, and cell culture supernatants. This second generation kit has increased sensitivity with a range from 8-1000pg/mL.
Brand: Invitrogen™ 88-8218-22
Alert:
Contains Sodium azide
Includes:
- Capture Antibody.Pre-titrated, purified antibody
- Detection Antibody.Pre-titrated, biotin-conjugated antibody
- Standard.Recombinant protein for generating standard curve and calibrating samples
- Coating Buffer.10X PBS ELISA Coating Buffer
- Assay Diluent.5X Concentrated
- Detection Enzyme.Pre-titrated Avidin-HRP
- Substrate Solution.Tetramethylbenzidine (TMB) Solution
- Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards.
Description
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6. As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function. IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells. In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23. While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3. IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules. In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.Specifications
Q9HBE4 | |
8 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
BSF-1,IL-4 | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
24 hr. 30 min. |
8-1,000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
59067 | |
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86) | |
2 x 96 Tests | |
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL | |
Interleukin-21 | |
1 hr. 45 min. |
For Research Use Only