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Invitrogen™ IL-29 Human ELISA Kit
For the quantitative detection of human IL-29.
£451.00 - £3070.00
Specifications
Accession Number | Q8IU54 |
---|---|
Assay Range | 15.6-1,000 pg/mL |
Assay Sensitivity | 2.0 pg/mL |
Conjugate | Biotin |
Product Type | ELISA Kit |
Includes: Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-29
Biotin-Conjugate anti-human IL-29 monoclonal antibody
Streptavidin-HRP
Human IL-29 Standard lyophilized, 2ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
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Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15561567
|
Invitrogen™
BMS2049 |
96 Tests |
£451.00
96 tests |
Estimated Shipment: 18-10-2024
Log in to see stock. |
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15571567
|
Invitrogen™
BMS2049TEN |
10 x 96 Tests |
£3070.00
Pack of 10 |
Estimated Shipment: 18-10-2024
Log in to see stock. |
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Description
The Human Interleukin-29 (Hu IL-29) ELISA quantitates Hu IL-29 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-29. Principle of the method The Human IL-29 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-29 is a protein of the helical cytokine family and is a type III interferon sharing many functions with the type I family of interferons. The IL-29 gene is found on chromosome 19 in humans. It is also known as IFN-lambda1 and is highly similar in amino acid sequence to the IL-28, the other type III interferon. IL-29 does not bind the IFN-alpha/beta receptor, but instead signals through a receptor composed of the IL-28R1 and IL-10R2 subunits. Initial characterizations of the receptor demonstrated that it has a ubiquitous tissue distribution and is expressed on most non-hematopoietic cells.
Generation of native IL-29 is achieved by monocytes and dendritic cells in response to viral infection and stimulation with toll-like receptor ligands. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL 29 has significant antiviral activity and immunoregulatory properties and appears to inhibit T helper-2 (Th2) responses regarding inhibition of IL-13 production, compared with IL-4 or IL-5. The antiviral activities of IL-29 include the upregulation of MHC Class I expression on the cell surface and the expression of PKR. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Specifications
Q8IU54 | |
2.0 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
IL-29,IL29 | |
7.6% | |
HRP | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
IFNL1, Interleukin 29 | |
1 hr. 20 min. |
15.6-1,000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
282618 | |
7.1% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
RUO | |
2°C to 8°C | |
Human | |
3 hr. 30 min. |
For Research Use Only