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Invitrogen™ IL-8 Human Instant ELISA™ Kit
Instant ELISA Kit
Brand: Invitrogen™ BMS204-3INST
Code : NEW
Additional Details : Weight : 0.23750kg
Includes:
- Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-8/NAP-1, Biotin-Conjugate (anti-human IL-8/NAP-1 polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
- Aluminum pouch(es) with a human IL-8/NAP-1 Standard curve (coloured)
- Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
- Sample Diluent (Use when an external predilution of the samples is needed)
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
- Adhesive Films
Description
The Human Interleukin 8 (Hu IL-8) ELISA quantitates Hu IL-8 in human serum, plasma, cell culture supernatants, amniotic fluid or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-8. Principle of the method The Human IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.Specifications
P10145 | |
1.3 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1 | |
8.1% | |
HRP | |
RUO | |
-20°C | |
Human | |
3 hr. 10 min. |
15.6-1,000 pg/mL | |
Biotin | |
Amniotic Fluid, Plasma, Serum, Supernatant | |
ELISA | |
3576 | |
10.8% | |
Pre-coated 96 well Microplate, Adhesive Plate Covers, SAV-HRP, SAV-HRP, lyophilized, Standard curve strips, Standard curve strips, Wash Buffer Concentrate, Stop Solution, Stop Solution, Lysis Buffer 10x | |
128 Tests | |
Amniotic Fluid, 50 μL; Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL | |
Protein 3-10C, CXCL8, NAP-1, GCP-1 | |
20 min. |
For Research Use Only