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Invitrogen™ TGFb1/LAP Human Uncoated ELISA Kit with Plates
Uncoated ELISA Kit with Plates
Brand: Invitrogen™ 88-50390-86
261.34 GBP valid until 2024-09-28
Use promo code "21626" to get your promotional price.
Alert:
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Includes:
- Capture Antibody Concentrate
- Detection Antibody Concentrate
- Human LAP standard (lyophilized): 20ng/mL upon reconstitution
- Phosphate Buffered Saline Concentrate (PBS)
- Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
- Sample Diluent B
- pre-titrated Streptavidin-HRP
- Tetramethylbenzidine (TMB) Substrate Solution
Description
The Human Transforming Growth Factor beta 1/LAP/CD276 Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TGFβ1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.Specifications
P01137 | |
156.3 pg/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
CED,DPD1,LAP,TGFB,TGFbeta | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
24 hr. 30 min. |
156.3-10,000 pg/mL | |
Biotin | |
Plasma, Serum, Supernatant | |
ELISA | |
7040 | |
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) | |
10 x 96 Tests | |
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL | |
Latency Associated Peptide, Pro-TGF beta 1, LAP/TGF beta 1 | |
1 hr. 45 min. |
For Research Use Only