Proteintech™ Human S100B ELISA Kit

KE00103 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100B ELISA kit is to be used to detect and quantify protein levels of endogenous S100B.

Brand:  Proteintech™ KE00103-96T

Product Code. 16811516

Description

• The assay recognizes human S100B. An antibody specific for S100B has been pre-coated onto the microwells. The S100B protein in samples is captured by the coated antibody after incubation.
• Following extensive washing, another antibody specific for S100B is added to detect the captured S100B protein.
• For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.
• Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
• S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain.
• It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte.
• Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage.
• An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).

Spécification

• Accession Number: NP_006263
• Assay Sensitivity: 1 pg/mL
• Detection Method: Sandwich ELISA, Double Antibody
• Sample Type: Plasma, Serum
• Target Species: Human
• Host Species: Human
• Interassay CV: <10%
• Shipping Condition: Blue ice
• Total Assay Time: 4.5 hr.

• Assay Range: 15.6 to 1000 pg/mL
• Certifications/Compliance: ISO 13485
• Immunoassay Kit Format: Coated ELISA Kit
• Substrate: DAB
• Gene ID (Entrez): 6285
• Immunoassay Kit Grade: Solid
• Intraassay CV: <10%
• Storage Requirements: -20°C to +4°C
• UniProt ID: P04271