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Invitrogen™ TNFR (Soluble-80 kDa) Human ELISA Kit
Provides a simple, rapid and highly sensitive method for the determination of soluble TNF-R (80 kd) levels in body fluids or cell culture supernatants.
Brand: Invitrogen™ BMS211
Includes:
- Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human sTNF-R (80 kDa)
- HRP-Conjugate anti-human sTNF-R (80 kDa) monoclonal antibody
- Human sTNF-R (80 kDa) Standard, 10ng/mL
- Control, lyophilized
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
- Substrate Solution (tetramethyl-benzidine)
- Stop Solution (1M Phosphoric acid)
- Blue-Dye
- Green-Dye
- Adhesive Films
Description
The Human Soluble Tumor Necrosis Factor Receptor (sTNFR-80 kDa) ELISA quantitates sTNFR in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sTNFR. Principle of the method The Human sTNFR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Tumor necrosis factor receptor 2 (TNFR2) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. This receptor plays an important role in mediating the metabolic effects of TNF-alpha. TNFR2 isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that TNFR2 regulates TNF-alpha function by antagonizing its biological activity.Specifications
P20333 | |
0.10 ng/mL | |
ELISA Kit | |
Human | |
Colorimetric Microplate Reader | |
CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR | |
1.4% | |
HRP | |
RUO | |
2°C to 8°C | |
Human | |
2 hr. 10 min. |
0.16-10.0 ng/mL | |
HRP | |
Plasma, Serum, Supernatant, Urine | |
ELISA | |
7133 | |
2% | |
Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Controls, Adhesive Plate Covers | |
96 Tests | |
Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL; Urine,10 μL | |
Tumor necrosis factor receptor 2, TNFR2, CD120b | |
1 hr. 20 min. |
For Research Use Only