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Invitrogen™ Human Wnt-1 Carrier-Free
Recombinant Protein
Brand: Invitrogen™ 34-8695-82
This item is not returnable.
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Description
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb. Description: Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb. Applications Reported: Recombinant human Wnt-1 is biologically active and can enhance BMP-induced alkaline phosphatase production by murine ATDC5 cells. Applications Tested: The ED50 of this protein, as determined by human BMP-2 (200ng/mL) induction of alkaline phosphatase production by murine ATDC5 cells, is 1.5-2.5ng/mL. Source: E. coli (amino acids Ala28-Leu370; accession # NP_005421). Bioactivity: The ED50 of this protein, as determined by human BMP-2 (200ng/mL) induction of alkaline phosphatase production by murine ATDC5 cells, is 1.5-2.5ng/mL. Endotoxin: Less than 0.1ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC. Molecular Weight: 42kDa (mature form). Storage and handling: For best recovery, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0mg/mL. Do not vortex. Use in sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.Specifications
P04628 | |
protein with no preservative | |
42kDa (mature form) | |
SDS-PAGE and HPLC | |
E. coli | |
RUO | |
Less than 0.1ng/μg cytokine as determined by the LAL assay | |
WNT1 | |
Recombinant Protein, Carrier-Free | |
Unconjugated | |
E. coli | |
Lyophilized | |
Affinity chromatography |
Functional Assay | |
7471 | |
Human Wnt-1 Carrier-Free | |
100μg | |
-20°C | |
E. coli (amino acids Ala28-Leu370; Accession # NP_005421) | |
Human Wnt-1 Carrier-Free | |
The ED50 of this protein, as determined by human BMP-2 (200ng/mL) induction of alkaline phosphatase production by murine ATDC5 cells, is 1.5-2.5ng/mL. | |
Human | |
Hu | |
Recombinant | |
Recombinant |