Hygromycin B, Thermo Scientific Chemicals

Description

Hygromycin B, CAS # 31282-04-9, is an aminoglycoside compound showing antibiotic activity that kills bacteria through the inhibition of protein synthesis. It also presents anthelmintic activity.

An aminoglycoside antibiotic that kills bacteria by inhibiting protein synthesisHygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells that contains the hygromycin resistance gene. This gene is frequently used as a selectable marker in research on plants. It stabilizes the transfer ribonucleic acid (tRNA)-ribosomal acceptor site, thereby inhibiting translocation. In addition to this, it serves as an aminoglycosidic antibiotic which inhibits protein synthesis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Hygromycin is an aminoglycosidic compound that shows antibiotic activity. It is produced by Streptomyces hygroscopicus
• Hygromycin acts against both prokaryotic and eukaryotic cells, having the ability to inhibit protein synthesis. It also stabilizes the transfer ribonucleic acid (tRNA)-ribosomal acceptor site, thereby inhibiting translocation. This compound prevents ribosome translocation during translation elongation by binding the 30S rRNA proximal to the ribosomal E, P, and A sites

Application

• In clinical and plant research, this compound can be used as a marker to select and maintain prokaryotic and eukaryotic cells containing the hygromycin resistance gene
• Hygromycin also presents an anthelmintic activity against nematode parasites of chickens

Specifications

Chemical Identifiers

• CAS: 31282-04-9
• Molecular Formula: C20H37N3O13
• Molecular Weight (g/mol): 527.524
• MDL Number: MFCD06795479
• InChI Key: GRRNUXAQVGOGFE-BBMONYMYSA-N
• PubChem CID: 134129613
• IUPAC Name: (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• SMILES: CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N

• Type: Hygromycin B
• Physical Form: Lyophilized Powder
• UN Number: UN3462
• Merck Index: 14,4852
• Solubility Information: Miscible with water,methanol,buffer solution and ethanol; Immiscible with less polar solvents.
• Formula Weight: 527.53
• Color: Brown
• Quantity: 100 mg
• Chemical Name or Material: Hygromycin B

RUO – Research Use Only