Hygromycin B, Thermo Scientific Chemicals

Description

Hygromycin B, CAS # 31282-04-9, is an aminoglycoside compound showing antibiotic activity that kills bacteria through the inhibition of protein synthesis. It also presents anthelmintic activity.

An aminoglycoside antibiotic that kills bacteria by inhibiting protein synthesisHygromycin B is used for the selection and maintenance of prokaryotic and eukaryotic cells that contains the hygromycin resistance gene. This gene is frequently used as a selectable marker in research on plants. It stabilizes the transfer ribonucleic acid (tRNA)-ribosomal acceptor site, thereby inhibiting translocation. In addition to this, it serves as an aminoglycosidic antibiotic which inhibits protein synthesis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Hygromycin is an aminoglycosidic compound that shows antibiotic activity. It is produced by Streptomyces hygroscopicus
• Hygromycin acts against both prokaryotic and eukaryotic cells, having the ability to inhibit protein synthesis. It also stabilizes the transfer ribonucleic acid (tRNA)-ribosomal acceptor site, thereby inhibiting translocation. This compound prevents ribosome translocation during translation elongation by binding the 30S rRNA proximal to the ribosomal E, P, and A sites

Application

• In clinical and plant research, this compound can be used as a marker to select and maintain prokaryotic and eukaryotic cells containing the hygromycin resistance gene
• Hygromycin also presents an anthelmintic activity against nematode parasites of chickens

Specifications

Chemical Identifiers

• CAS: 31282-04-9
• Molecular Formula: C20H37N3O13
• Molecular Weight (g/mol): 527.524
• MDL Number: MFCD06795479
• InChI Key: GRRNUXAQVGOGFE-BBMONYMYSA-N
• PubChem CID: 134129613
• IUPAC Name: (3'S,3aS,4S,4'S,5'R,6R,6'R,7R,7aS)-4-[(1R,2S,3R,5S,6S)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
• SMILES: CNC1CC(C(C(C1O)OC2C3C(C(C(O2)CO)O)OC4(O3)C(C(C(C(O4)C(CO)N)O)O)O)O)N

• Type: Hygromycin B
• Physical Form: Lyophilized Powder
• UN Number: UN3462
• Merck Index: 14,4852
• Solubility Information: Miscible with water,methanol,buffer solution and ethanol; Immiscible with less polar solvents.
• Formula Weight: 527.53
• Color: Brown
• Quantity: 250 mg
• Chemical Name or Material: Hygromycin B

Safety and Handling

Product Identifier

• Hygromycin B

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 1
• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 1
• Respiratory sensitiser Category 1
• Skin sensitiser Category 1

Hazard Statement

• H300-Fatal if swallowed.
• H310-Fatal in contact with skin.
• H317-May cause an allergic skin reaction.
• H318-Causes serious eye damage.
• H330-Fatal if inhaled.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.

Supplemental information

• MIXTURE LIST-Contains : Hygromycin B

RUO – Research Use Only