Invitrogen™ Ambion™ mirVana™ miRNA Inhibitor, let-7c positive control

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

Product Code	Brand	Quantity
13315384	Invitrogen™ 4464080	5 nmol
13325384	Invitrogen™ 4464081	20 nmol

Description

miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

We have now released the second generation of miRNA inhibitors, mirVana™ miRNA Inhibitors, which:

• Display better potency than lead competitor's product
• Offer maximum and consistent effect in vitro at low miRNA inhibitor concentration
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering consistency throughout your entire research project
• Are non-toxic

Positive and negative controls are also available for use along with mirVana™ miRNA Inhibitors in miRNA down-regulation experiments.

mirVana™ miRNA Inhibitor let-7c Positive Control

The mirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments using mirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.

First Generation Inhibitor Controls

Our first-generation miRNA inhibitors (and controls), Ambion™ Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Inhibitors and their corresponding controls.

Specifications

• Content And Storage: Store at or below -20°C. Do not store in a frost-free freezer.
• RNAi Type: miRNA Inhibitor
• Label or Dye: Unconjugated
• Purification Method: Purified

• Control Type: Positive Control
• For Use With (Application): miRNA & Non-Coding RNA Analysis
• Product Type: miRNA Inhibitor
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.