Invitrogen™ Pichia Expression Kit, original kit

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.

Brand:  Invitrogen™ K171001

Product Code. 10768244

Description

The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris. The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium. The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.

Specifications

• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Content And Storage: The Original Pichia Expression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression, Pichia pastoris strains GS115 (his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5' AOX1, 3' AOX1, and α-factor sequencing primers.
  
  Store vectors and primers at -20°C. Store strains at room temperature.
  All components are guaranteed stable for 6 months when properly stored., Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months
• Promoter: AOX1
• Format: Kit
• Vector: pPIC
• Expression Mechanism: Cell-Based Expression
• For Use With (Application): Protein Expression
• Protein Tag: Untagged

• Bacterial or Yeast Strain: GS115, KM71
• Cloning Method: Restriction Enzyme⁄ MCS
• Cell Type: Yeast Cells
• Species: P. pastoris
• Selection Agent (Eukaryotic): Ampillicin
• Expression System: Yeast
• Product Type: Expression Kit
• Quantity: 1 Kit

For Research Use Only. Not for use in diagnostic procedures.