Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Product Code	Brand	No. of Reactions
10734227	Applied Biosystems™ 4391178	40 Reactions
10229194	Applied Biosystems™ 4389986	200 Reactions

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

• Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
• Formulated to ensure maximum sensitivity and reliability
• Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

• AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

Specifications

• Detection Method: SYBR
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Passive Reference Dye: ROX (Pre-mixed)
• Purity or Quality Grade: UP (Ultra Pure)
• Run Time: Standard
• For Use With (Application): Gene Expression

• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Label or Dye: SYBR Green
• Product Line: RNA-to-C_T™, SYBR™
• Reverse Transcriptase: ArrayScript™ UP
• Shipping Condition: Wet Ice
• Sample Type: RNA

For Research Use Only. Not for use in diagnostic procedures.