ICAM-1 Monoclonal Antibody (3E2B), Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Invitrogen MA5405

Product Code. 11834311

Description

MA5405 targets CD54 in WB, ELISA, FACS, IA, IHC, ICC/IF and Neu applications and shows reactivity with mouse samples. The MA5405 immunogen is mouse ICAM-1 (CD54). MA5405 detects CD54 which has a predicted molecular weight of approximately 56 kDa. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

Specifications

• Antigen: ICAM-1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P13597
• Gene Symbols: ICAM1
• Immunogen: Mouse ICAM-1 (CD54)
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Inhibition Assays, Neutralization, Western Blot
• Clone: 3E2B
• Conjugate: Unconjugated
• Gene: ICAM1
• Gene Alias: BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
• Host Species: Armenian Hamster
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 15894
• Content And Storage: -20°C
• Form: Liquid