Invitrogen™ IgG2A Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMIGG2AX5

Product Code. 17195739

Description

The Mouse Immunoglobulin 2A (Ms Ig2A) ELISA quantitates Ms Ig2A in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Ig2A. Principle of the method The Mouse Ig2A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Specifications

• Assay Range: 0.614 ng/mL to 150 ng/mL
• Calibration Range: 0.6 ng/mL to 150 ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• Specificity: This ELISA pair antibody detects mouse heavy chain of IgG2a. Other species not determined.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 2 μ;L; Serum, 2 μ;L; Supernatant, 100 μ;L
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 0.6 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Interassay CV: <12%
• Kit Contents: Assay Diluent Concentrate, Adhesive Plate Covers, Pre-coated 96 well plates, Biotinylated Detection Antibody, SAV-HRP, Standard, Stop Solution, Wash Buffer, Chromogen
• Quantity: 5 x 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.