Invitrogen™ IL-12 (IL-12A) Porcine ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15415403	Invitrogen™ ESIL12A	96 Tests
17105979	Invitrogen™ ESIL12AX5	5 x 96 Tests

Description

The Porcine Interleukin 12 (IL-12A) ELISA quantitates Porc IL-12A in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-12A. Principle of the method The Porcine IL-12A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) encoded by the IL12B gene and 35-kD (p35) encoded by the IL12A gene. IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-γ) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-γ and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

Specifications

• Accession Number: Q28938,Q29053
• Calibration Range: 2.2 ng/ml to 600 ng/ml
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL12A, IL12B
• Intraassay CV: <10%
• Label or Dye: HRP
• Research Discipline: Oncology
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 2.2 ng/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 397053, 397076
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Target: Porcine
• Total Assay Time: 4 hr. 45 min.