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IL-12/IL-23 p40 Monoclonal Antibody (eBioHP40 (HP40, HP-40)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 46-7235-42
Description
Description: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope. Applications Reported: This eBioHP40 (HP40, HP-40) antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.Specifications
IL-12/IL-23 p40 | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P29459, P29460, Q9NPF7 | |
IL12A, IL12B, Il23a | |
Affinity chromatography | |
RUO | |
3592, 3593, 51561 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBioHP40 (HP40, HP-40), HP-40) | |
PerCP-eFluor 710 | |
IL12B | |
CLMF; CLMF p35; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; Il12; Il-12 p35; IL12 p40; IL23; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il12a; IL-12A; Il12b; IL-12B; IL12p35; IL-12p35; Il12p40; Il-12p40; il23; IL-23; IL23 p41; IL-23 subunit alpha; Il23a; IL-23A; IL-23-A; IL23P19; IL-23p19; IL35 subunit; ILN; IMD28; IMD29; Interleukin; Interleukin 12; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 polypeptide 35; interleukin 12 subunit p40; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12b; interleukin 12B (natural killer cell stimulatory f | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.