Invitrogen™ IL-13 Porcine ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ ESIL13X5

Product Code. 17115979

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Porcine Interleukin 13 (IL-13) ELISA quantitates Porc IL-13 in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc IL-13. Principle of the method The Porcine IL-13 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

Specifications

• Accession Number: Q95J68
• Assay Sensitivity: 16 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 96 assays
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-13,L13
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 20 μL; Serum, 20 μL; Supernatant, 50 μL
• Synonym: IL-13L13
• Test Time: 1 hr. 20 min.

• Assay Range: 20.58 pg/mL to 15,000 pg/mL
• Calibration Range: 16 pg/mL to 15000 pg/mL
• Conjugate: Biotin
• Protein Family: Cytokines & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: porcine CCL3L1, IFN alpha, IL-1 alpha, IL-1 R alpha, IL-17 A, IL-18, MIG, MIP-1 beta, PECAM-1.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 396721
• Interassay CV: <12%
• Kit Contents: Chromogen, Adhesive Plate Covers, Assay Diluent concentrate, Stop Solution, Wash Buffer, Standard, Biotinylated Detection Antibody, Pre-coated 96 well plate, SAV-HRP
• Quantity: 5 x 96 Tests
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Porcine
• Total Assay Time: 4 hr. 45 min.