Invitrogen™ IL-2 Bovine ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15444873	Invitrogen™ EBIL2	96 Tests
17756789	Invitrogen™ EBIL2X10	10 x 96 Tests
17144789	Invitrogen™ EBIL2X5	5 x 96 Tests
17844040	Invitrogen™ EBIL2X10	10 x 96 Tests

Description

The Bovine Interleukin 2 (Bov IL-2) ELISA quantitates Bov IL-2 in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov IL-2. Principle of the method The Bovine IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Accession Number: P05016
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL2
• Intraassay CV: <10%
• Label or Dye: HRP
• Research Discipline: Immunology
• Storage Requirements: 2°C to 8°C
• Target: Bovine
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 0.2 ng/mL
• Conjugate: Biotin
• Protein Family: Other Proteins
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 280822
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
• Synonym: BOS_16374, IL-2, TCGF
• Test Time: 1 hr. 20 min.